Establishment of the in Vitro dose-Response Calibration Curve for X-ray-induced Micronuclei in Human Lymphocytes

Radiat Prot Dosimetry. 2022 Aug 13:ncac170. doi: 10.1093/rpd/ncac170. Online ahead of print.

ABSTRACT

The cytokinesis-block micronucleus assay has proven to be a reliable technique for biological dosimetry. This study aimed to establish the dose-response curve for X-ray-induced micronucleus. Peripheral blood samples from three healthy donors were irradiated with various doses and scoring criteria by the micronuclei (MN) in binucleated cells. The results showed that the frequency of MN increased with the elevation of radiation dose. CABAS and Dose Estimate software were used to fit the MN and dose into a linear quadratic model, and the results were compared. The linear and quadratic coefficients obtained by the two software were basically the same and were comparable with published curves of similar radiation quality and dose rates by other studies. The dose-response curve established in this study can be used as an alternative method for in vitro dose reconstruction and provides a reliable tool for biological dosimetry in accidental or occupational radiation exposures.

PMID:35961020 | DOI:10.1093/rpd/ncac170

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